Anti-KMT1E / SETDB1 antibody ab12317 from Abcam

Product Specs
ItemAnti-KMT1E / SETDB1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab12317
Antibody TypePrimary
ClonalityPolyclonal
HostRabbit
ApplicationsWestern blot
IsotypeIgG
Quantity50 UG
Conjugate/TagUnconjugated
FormatPurified using the peptide immobilized on solid support
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameSETDB1
ReactivityHuman
TargetKMT1E / SETDB1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancersMolecular cellFebruary 17, 2022Barral A, Pozo G, Ducrot L, Papadopoulos GL, Sauzet S, Oldfield AJ, Cavalli G, Déjardin J.Anti-Histone H3 (tri methyl K9) - ChIP Grade,Abcam,Cat#ab8898; RRID:AB_306848 Anti-KMT1E / SETDB1 antibody,Abcam,Cat#ab12317; RRID:AB_299005 "Rabbit IgG, polyclonal – Isotype Control Grade",Abcam,Cat#ab171870; RRID:AB_2687657 "Rabbit

(2) WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathwayJournal of Experimental MedicineJanuary 3, 2022Ensong Guo et al.1:200); Foxp3 antibody (Cell Signaling Technology; cat #12653; 1:200); SETDB1 antibody (Abcam; cat #ab12317; 1:200); and FOXM1 antibody (Abcam; cat #ab232649; 1:200). Tissue sections were then stained using an

(3) Histone Methyltransferase SETDB1 Promotes Immune Evasion in Colorectal Cancer via FOSB-Mediated Downregulation of MicroRNA-22 through BATF3/PD-L1 PathwayJournal of immunology researchJanuary 1, 2022Tian J, Wang W, Zhu J, Zhuang Y, Qi C, Cai Z, Yan W, Lu W, Shang A.promoter region using ChIP kit (17371; Millipore, Billerica, MA, USA) [20] with rabbit antibody to SETDB1 (ab12317, 1 : 500, Abcam) and H3K9me3 (ab8898, 1 : 200, Abcam). The promoter sequence of FOSB in the complex

(4) A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH ComplexACS chemical biologySeptember 17, 2021Waybright JM, Clinkscales SE, Barnash KD, Budziszewski GR, Rectenwald JM, Chiarella AM, Norris-Drouin JL, Cholensky SH, Pearce KH, Herring LE, et al.SC398562, IB), CDYL2 (Abcam, ab183854), HMGN2 (Abcam, ab199679), TRM28 (Abcam, ab10484), SETDB1 (Abcam, ab12317), MYC (Millipore Sigma, 05–724), FLAG (Abcam, 205606), HP1α (Abcam, ab77256, IP), HP1β (Abcam, ab10811,

(5) JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stabilityEpigenetics & ChromatinDecember 1, 2020Francisco Saavedra, Zachary A. Gurard-Levin, Camila Rojas-Villalobos, Isabelle Vassias, Raquel Quatrini, Geneviève Almouzni, Alejandra Loyola(Cell Signaling Technology 2621S), NASP (Dr. Almouzni), Topo I (Santa Cruz sc-32736), SetDB1 (Abcam ab12317). Extract preparation HeLa cells were cultured in Dulbecco’s modified Eagle medium (DMEM) (GE healthcare

(6) Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced DamageCellMay 14, 2020Nava MM, Miroshnikova YA, Biggs LC, Whitefield DB, Metge F, Boucas J, Vihinen H, Jokitalo E, Li X, García Arcos JM, et al.1:2500), Lamin B1 (Cell Signaling 9087; 1:1000), Lamin B2 (Cell Signaling 9622; 1:1000), Setdb1 (Abcam ab12317; 1:2000), Suv39h1 (Thermo PA-29470; 1:2000), pLamin A (S22; Invitrogen PA5-17113; 1:2500), pPaxillin

(7) The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin stateScientific ReportsSeptember 1, 2016Valentina Alarcon, Sergio Hernández, Lorena Rubio, Francisca Alvarez, Yvo Flores, Manuel Varas-Godoy, Giancarlo V. De Ferrari, Michael Kann, Rodrigo A. Villanueva, Alejandra Loyola(Abcam #ab17721), anti-FLAG beads (Sigma Aldrich #A2220), Set1A (Bethyl #A300-290A), SetDB1 (Abcam #ab12317). Drugs: Pargyline hydrochloride (sc-215676A, Santa Cruz Biotechnology). Primer cccDNA: Forward 5′-ACTCTTGGACTTTCAGGAAGG-3,

(8) Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposonsProceedings of the National Academy of Sciences of the United States of AmericaNovember 24, 2015Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama EN, Ohkawa Y, Tsukada Y, Ogura A.

(9) Methylation of histone H3 lysine 9 occurs during translationNucleic acids researchOctober 30, 2015Rivera C, Saavedra F, Alvarez F, Díaz-Celis C, Ugalde V, Li J, Forné I, Gurard-Levin ZA, Almouzni G, Imhof A, et al.A300–195A), NASP (donated by Dr Almouzni), RPL5 (Abcam #ab74744), RPS3a (Abcam, ab171742), SetDB1 (Abcam #ab12317). For Western blot analysis the primary antibodies were detected with a horseradish peroxidase-conjugated

(10) AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor geneNucleic Acids ResearchDecember 16, 2014Sunwha Cho, Jung Sun Park, Yong-Kook KangMETHODS Antibodies The list of antibodies we used was as follows: anti-SETDB1 (Upstate 07-378 for NT and Abcam ab12317 for CT), -DICER1 (sc-30226, Santa Cruz), -AGO2 (2897, Cell signaling), -AR (3202, Cell signaling), -PR

(11) SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and ageNature CommunicationsDecember 1, 2014Michael Van Meter, Mehr Kashyap, Sarallah Rezazadeh, Anthony J. Geneva, Timothy D. Morello, Andrei Seluanov, Vera Gorbunovaused in this study: 1:500 SIRT6 (ab48352, ab62739; Abcam), 1:500 KAP1 (ab10483, Abcam), 1:500 SETDB1 (ab12317, Abcam), 1:500 MeCP2 (ab2828, Abcam), 1:1000 H3K9me3 (ab8898, Abcam), 1:1000 HP1α (mab3448, Millipore). Luciferase

(12) Ectopic DNMT3L Triggers Assembly of a Repressive Complex for Retroviral Silencing in Somatic CellsJournal of VirologySeptember 15, 2014Tzu-Hao Kao et al.Cheng), antiTRIM28 polyclonal (ab10484; Abcam), anti-HDAC1 polyclonal (ab7028; Abcam), anti-SETDB1 (ab12317; Abcam), anti-HP1␥ (ab10480; Abcam), and anti-EZH2 (17-662; Millipore). For immunofluorescence staining,

(13) Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1Nucleic acids researchJune 1, 2014Calderon MR, Verway M, Benslama RO, Birlea M, Bouttier M, Dimitrov V, Mader S, White JH.Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for HA (ab1424), ZBRK1 (ab77085), KAP-1 (ab10483) and SETDB1 (ab12317) were purchased from Abcam (Cambridge, MA, USA). Antibodies for FLAG (F1804) and FGF2 (F5180) were purchased

(14) Gene amplification of the histone methyltransferase SETDB1 contributes to human lung tumorigenesisOncogeneMay 22, 2014Rodriguez-Paredes M, Martinez de Paz A, Simó-Riudalbas L, Sayols S, Moutinho C, Moran S, Villanueva A, Vázquez-Cedeira M, Lazo PA, Carneiro F, et al.reverse transcription–PCR (c) and western blot (d) demonstrate higher levels of SETDB1 mRNA and protein (ab12317, Abcam, Cambridge, UK), respectively, in amplified cancer cell lines (H1437, NCI-H1395 and DMS-273) than

(15) Regulated nuclear entry of over-expressed Setdb1Genes to cells : devoted to molecular & cellular mechanismsAugust 1, 2013Sunwha Cho, Jung Sun Park, Yong-Kook Kang

(16) SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage responseNucleic acids researchDecember 1, 2012Mund A, Schubert T, Staege H, Kinkley S, Reumann K, Kriegs M, Fritsch L, Battisti V, Ait-Si-Ali S, Hoffbeck AS, et al.HP1-γ (ab10817; Abcam), Tif1-β/KAP-1 (MAB3662; Millipore), pKAP-1-S824 (A300-767A-1; Bethyl), SETDB1 (ab12317; Abcam), SETDB1 (A300-121A; Bethyl), SUV39H1 (05-615; Upstate), SUV39H1 (07-550; Upstate). SPOC1 was

(17) Jarid2 (Jumonji, AT rich interactive domain 2) regulates NOTCH1 expression via histone modification in the developing heartThe Journal of biological chemistryJanuary 6, 2012Matthew R Mysliwiec, Clayton D Carlson, Josh Tietjen, Holly Hung, Aseem Z Ansari, Youngsook Lee

(18) Dual functions of histone-lysine N-methyltransferase Setdb1 protein at promyelocytic leukemia-nuclear body (PML-NB): maintaining PML-NB structure and regulating the expression of its associated genesThe Journal of biological chemistryNovember 25, 2011Sunwha Cho, Jung Sun Park, Yong-Kook Kang

(19) The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onsetNatureMarch 24, 2011Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, Battisti V, Fritsch L, Lin WM, Hollmann TJ, Ferré F, et al.

(20) A SUMO-regulated activation function controls synergy of c-Myb through a repressor-activator switch leading to differential p300 recruitmentNucleic acids researchAugust 1, 2010Molvaersmyr AK, Saether T, Gilfillan S, Lorenzo PI, Kvaløy H, Matre V, Gabrielsen OS.antibody (F3165, Sigma), isotype control: anti-secretory Component (IgA) IgG1 (I6635, Sigma), anti-SETDB1 (ab12317-50, Abcam), anti-Histone H3 trimethylated at lysine 9 (pAb-056-050, Diagenode), anti-Mi2 (06-878, Millipore

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Anti-KMT1E / SETDB1 antibody from Abcam

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Anti-KMT1E / SETDB1 antibody from Abcam

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